98th Annual Meeting DOG 2000

V 595

Effect of Prostaglandin F2a on corneal endothelial proliferation in vitro

P. Rieck, B. Edelmann, S. Schmidt, C. Hartmann

Introduction: Prostaglandines, especially PGF2a, have recently been introduced as a new local glaucoma medication. The modulation of cell proliferation and collagen synthesis in various tissues are major effects of these agents. However, up to now it remains unknown whether PGF2a modulates also the proliferation of the corneal endothelium.

Methods: Bovine corneal endothelial cells (BCEC) were cultured according to established protocols. Experiments were performed after the 1. passage under serum reduced conditions. 104 cells/well were seeded and the cells were then incubated with different (5x10-5 - 5x10-3 mg/ml) concentrations of PGF2a (Sigma). The number of cells was determined every 24h until day 5. Toxicity tests were performed by means of the trypan blue exclusion assay.

Results: With all concentrations tested, PGF2a induced a significant stimulation of BCEC proliferation. At the highest concentration of PGF2a, a 2 fold increase in cell number was found after 5 days only compared to unsupplemented control cultures. No signs of cellular toxicity or morphological alterations could be detected in PGF2a -supplemented cells.

Discussion: For the first time, the present study demonstrates a stimulatory effect of PGF2a on the corneal endothelium. It appears interesting that this effect is also induced by the PGF2a concentration determined in the aqueous humor after local application. However, due to the strong contact inhibition of endothelial cells in situ, these results cannot be directly extrapolated to the situation in patients with local latanoprost treatment.

Dpt. of Ophthalmology, Charité, Campus Virchow Klinikum, Augustenburger Platz 1, D13353 Berlin