98th Annual Meeting DOG 2000

K 42

Amniotic membrane has anti-inflammatory properties

F. E. Kruse, L. You, S. Ebner, K. Rohrschneider, H. E. Völcker

Transplantation of preserved amniotic membrane (AMT) is becoming a valuable technique for reconstruction of the ocular surface. However the mechanism is largely unknown. We investigated the hypothesis that amniotic membrane has an effect on ocular inflammation

Methods: In tissue culture keratocytes were stimulated with inflammatory mediators. mRNA for various CXC chemokines was quantified by dot and northern blots.

Results: Clinically, AMT caused a consistent reduction of inflammation when applied to corneal epithelial defects and ulcers. In vitro keratocytes seeded on amniotic membranes showed less expression of chemokines (Il-8, gro-alpha, ENA-78) compared to cells seeded on plastic.

Discussion: AMT is a valuable tools for ocular surface reconstruction. Our findings offer a mechanism by which amniotic membrane suppresses inflammation when transplanted to the surface of the eye.

Augenklinik der Universität Heidelberg, INF 400, D-69120 Heidelberg



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