K 39Surfacemodification of silicone to improve human corneal keratocytes adhesion
E. Berger1, K. Hahn1, J. Saedler1, C. F. Kreiner2
Purpose: Silicone is planned to use as keratoprosthesis optic . The adhesion of keratocytes is prerequisite in order to guarantee a stabilization of keratoprosthesis in the cornea. We investigated different surface-modifications of the hydrophobic material regarding the improvement of the keratocytes adhesion.
Materials and Methods: In-vitro examinations of untreated and with ammonia-plasma as well as additional collagen type I coating surface-modified silicone were enforced in tissue culture with human keratocytes. Superficial cellgrowth and cellstructure were examined over a time period of 7 days light-microscopically.
Results: Adhesion of corneal keratocytes on the different surfaces already became visible a hour after the passage. While no keratocytes proliferation could be documented on the untreated silicone, round suspensioncells lay beside the material. On plasma-modified material, the keratocytes were already adherent and had begun with spreading. After 7 days cultivation opposed the confluent celllayer on modified surface with the low growth of single keratocytes on untreated silicone .
Discussion: After positive results of in-vitro investigation of surface-modified silicone further examinations in-vivo are necessary. The manufacture of an adhesive interface between silicone-optics and corneal stroma is impeded by the low quantity of keratocytes in the animal cornea. Therefore additional to the plasma-modified silicone type I collagen coating , that showed in-vitro no advantages, could be helpful in the animal experiment.
1Dept. of Ophthalmology, University of Rostock, Doberaner-Str. 140, D-18057 Rostock, Germany